{"product_id":"wdr5-s22-c334","title":"Recombinant \u003cb\u003eWDR5\u003c\/b\u003e(S22-C334), WD40 repeat-containing protein 5","description":"\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"UTF-8\"\u003e \u003cmeta name=\"viewport\" content=\"width=device-width, initial-scale=1.0\"\u003e\u003c\/p\u003e\n\u003cstyle\u003e\n    \/* 1. VARIABLEN \u0026 RESET *\/\n    :root {\n        --primary-color: #0dbaad;\n        --text-dark: #373636;\n        --border-light: #e0e0e0;\n    }\n\n    * { margin: 0; padding: 0; box-sizing: border-box; }\n\n    .container {\n        max-width: 1200px;\n        margin: 0 auto;\n        background-color: white;\n        padding: 20px;\n        font-family: \"Source Sans Pro\", sans-serif;\n        color: var(--text-dark);\n        line-height: 1.6;\n    }\n\n    \/* 2. 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MOBILE ANPASSUNGEN *\/\n    @media (max-width: 600px) {\n        .product-top-section {\n            flex-direction: column;\n            align-items: flex-start; \/* List-Items linksbündig *\/\n        }\n        .image-column {\n            order: -1;\n            margin-bottom: 10px;\n            width: 100%;\n            justify-content: center;\n        }\n        .side-image {\n            margin-top: 0;\n            max-width: 150px;\n        }\n        .custom-list { font-size: 19px; }\n        .accordion-header { font-size: 20px; }\n    }\n\n  \/* 6. INTRO TEXT SEKTION *\/\n    .intro-section {\n        margin: 20px 0;\n        font-size: 19px; \/* Desktop Größe *\/\n        line-height: 1.8;\n    }\n\n    \/* Mobile Anpassung für den Intro-Text *\/\n    @media (max-width: 600px) {\n        .intro-section {\n            font-size: 17px; \n            text-align: left;\n            padding: 0 5px;\n        }\n    }\n\u003c\/style\u003e\n\u003cdiv class=\"container\"\u003e\n\u003cdiv class=\"product-top-section\"\u003e\n\u003cdiv class=\"info-column\"\u003e\n\u003cul class=\"custom-list\"\u003e\n\u003cli\u003eCrystallizable\u003c\/li\u003e\n\u003cli\u003eSmartSoak  \u003csup\u003e®\u003c\/sup\u003e   established\u003c\/li\u003e  \n\u003cli\u003ePurity \u0026gt; 95%\u003c\/li\u003e\n\u003cli\u003eHighly monodisperse\u003c\/li\u003e\n\u003cli\u003eMelting temperature of 65°C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"image-column\"\u003e\u003cimg class=\"side-image\" alt=\"Quality Plus Siegel\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/Stempel-QUALITY-PLUS-new.png?v=1771953936\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003chr style=\"margin: 30px 0; height: 2px; background-color: #0dbaad; border: none;\"\u003e\n\u003cdiv class=\"intro-section\"\u003e\n\u003cp\u003eWD40-repeat-containing protein 5 (WDR5) is a highly conserved scaffold protein that is capable of a variety of different regulatory functions by forming complexes with a diverse range of WDR5-interacting proteins. It plays a critical role in histone modification and chromatin remodeling as well as muscle homeostasis, making it a pivotal target in the development of therapeutic strategies for cancer and muscle degenerative diseases.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-container\"\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003econstruct design \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003eOur truncated human WDR5 (S22 - C334) construct enables sufficient expression of soluble and stable protein in \u003cem\u003eE. coli.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eProtein Properties \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003e\u003cb\u003eProtein Construct:\u003c\/b\u003e S22-C334\u003cbr\u003e\u003cb\u003eSource:\u003c\/b\u003e Human\u003cbr\u003e\u003cb\u003eExpression Host:\u003c\/b\u003e \u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cbr\u003e\u003cb\u003eMolecular weight [kDa]:\u003c\/b\u003e 34.35\u003cbr\u003e\u003cb\u003epI:\u003c\/b\u003e 8.64\u003cbr\u003e\u003cb\u003eExtinction Coefficient [M\u003csup\u003e-1\u003c\/sup\u003ecm\u003csup\u003e-1\u003c\/sup\u003e]:\u003c\/b\u003e 68410\u003cbr\u003e\u003cb\u003eConcentration [mg\/mL]:\u003c\/b\u003e 10.57\u003cbr\u003e\u003cb\u003eStorage Buffer:\u003c\/b\u003e 20 mM HEPES pH 7.0, 250 mM NaCl, 5 mM DTT\u003cbr\u003e\u003cb\u003eTag:\u003c\/b\u003e none\u003c\/p\u003e\n\u003cp style=\"margin-top: 15px;\"\u003e\u003cb\u003eAmino acid sequence:\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"sequence-box\"\u003eSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eQuality Plus \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003e\u003cb\u003eQC Data:\u003c\/b\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/WDR5_datasheet.pdf?v=1772444916\" target=\"_blank\"\u003eDatasheet\u003c\/a\u003e\u003cbr\u003e\u003cb\u003ePurity SDS:\u003c\/b\u003e \u0026gt;95 % \u003cbr\u003e\u003cb\u003eHydrodynamic Radius (nm):\u003c\/b\u003e 2.49\u003cbr\u003e\u003cb\u003ePolydispersity Index (PDI): \u003c\/b\u003e 0.03\u003cbr\u003e\u003cb\u003eT\u003csub\u003eon\u003c\/sub\u003e (°C):\u003c\/b\u003e 53.7\u003cbr\u003e\u003cb\u003eT\u003csub\u003em\u003c\/sub\u003e (°C):\u003c\/b\u003e 65.4\u003cbr\u003e\u003cb\u003eCalculated Mass [Da]:\u003c\/b\u003e 34325.368 \u003cbr\u003e\u003cb\u003eMeasured Mass [Da]:\u003c\/b\u003e 34323.39\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eScientific context \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003ch4\u003eWDR5 Structure drives function diversity\u003c\/h4\u003e\n\u003cp\u003eWDR5 is well known for acting as a scaffold protein that enables the assembly of numerous complexes. Its highly conserved β-propeller structure gives WDR5 its toroidal shape, which is essential for its function, as it features the \"WDR5-binding\" (WBM) motif on one side and the \"WDR5-interacting (WIN) motif on the other surface. These sites enable the engagement of WDR5 in nearly 200 different protein interactions.\u003c\/p\u003e\n\u003ch4\u003eKey functions\u003c\/h4\u003e\n\u003cp\u003eWDR5 plays an important role in chromatin modification: It is able to catalyze H3K4 methylation via complex formation with MLL\/SET1, which is typically associated with gene activation or enforce gene silencing by complexation with NuRd. These versatile functions make WDR5 a key player in epigenetic regulation across different tissues and cellular contexts. \u003cbr\u003eOne main regulatory process involving WDR5 is maintenance of muscle homeostasis: Skeletal muscle regeneration relies on muscle-specific stem cells, or satellite cells, which express the transcription factor PAX7 to drive adult myogenesis. PAX7 recruits WDR5 and the MLL complex to regulatory regions of Myf5, promoting chromatin accessibility, H3K4me3 enrichment, and Myf5 expression. This highlights H3K4 methylation as a key aspect of WDR5 function, as loss of WDR5 significantly reduces H3K4me levels at specific muscle gene promoters.\u003c\/p\u003e\n\u003ch4\u003eOncogenic Role\u003c\/h4\u003e\n\u003cp\u003eIn cancers driven by MLL fusion proteins (a common cause of aggressive leukemia), WDR5 is co-opted to drive aberrant H3K4 methylation and constitutive activation of pro-leukemic genes. Its dual degradation together with the Ikaros zinc finger (IKZF) transcription factor is a promising anti-cancer strategy.\u003c\/p\u003e\n\u003ch4\u003eTargeting Strategy\u003c\/h4\u003e\n\u003cp\u003eSmall molecule inhibitors that successfully compete with the H3 peptide for binding in the central pocket of WDR5 are powerful tools for disrupting the complex's ability to methylate H3K4, offering a promising therapeutic approach for MLL-rearranged leukemias.\u003c\/p\u003e\n\u003cdiv style=\"font-size: 15px; margin-top: 25px; border-top: 1px dotted #ccc; padding-top: 15px;\"\u003e\n\u003cb\u003eReferences:\u003c\/b\u003e\u003cbr\u003eEpigenetics. 2025 Oct 13; 20(1):2573998. doi:\u003ca href=\"https:\/\/doi.org\/10.1080\/15592294.2025.2573998\" target=\"_blank\"\u003e10.1080\/15592294.2025.2573998\u003c\/a\u003e \u003cbr\u003eOncogene. 2022 May 07, 413328–3340. doi:\u003ca href=\"https:\/\/www.nature.com\/articles\/s41388-022-02340-8\" target=\"_blank\"\u003e10.1038\/s41388-022-02340-8\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"CrystalsFirst Shop","offers":[{"title":"1 mg \/ No tag","offer_id":56479681773951,"sku":null,"price":1750.0,"currency_code":"EUR","in_stock":true},{"title":"1 mg \/ AVI-Tag (biotinylated)","offer_id":56469274722687,"sku":null,"price":2250.0,"currency_code":"EUR","in_stock":false},{"title":"5 mg \/ No tag","offer_id":56479681839487,"sku":null,"price":7850.0,"currency_code":"EUR","in_stock":true},{"title":"5 mg \/ AVI-Tag (biotinylated)","offer_id":56469274755455,"sku":null,"price":8850.0,"currency_code":"EUR","in_stock":false},{"title":"10 mg \/ No tag","offer_id":56479681905023,"sku":null,"price":14000.0,"currency_code":"EUR","in_stock":true},{"title":"10 mg \/ AVI-Tag (biotinylated)","offer_id":56469274788223,"sku":null,"price":16000.0,"currency_code":"EUR","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/webshopbilder.WDR5_47858c4b-f3f0-41b3-913a-9eea8806124a.jpg?v=1774045186","url":"https:\/\/shop.crystalsfirst.com\/products\/wdr5-s22-c334","provider":"CrystalsFirst GmbH","version":"1.0","type":"link"}