{"product_id":"soon-available-br-recombinant-b-e6ap-b-konstukt-x-domain-voller-name","title":"Recombinant \u003cb\u003eE6AP\u003c\/b\u003e(Q495-L852), HECT domain, E3 Ligase, E6-associated protein, UBE3A","description":"\u003cp\u003e\u003cmeta charset=\"UTF-8\"\u003e \u003cmeta content=\"width=device-width, initial-scale=1.0\" name=\"viewport\"\u003e\u003c\/p\u003e\n\u003cstyle\u003e\n    \/* 1. VARIABLEN \u0026 RESET *\/\n    :root {\n        --primary-color: #0dbaad;\n        --text-dark: #373636;\n        --border-light: #e0e0e0;\n    }\n\n    * { margin: 0; padding: 0; box-sizing: border-box; }\n\n    .container {\n        max-width: 1200px;\n        margin: 0 auto;\n        background-color: white;\n        padding: 20px;\n        font-family: \"Source Sans Pro\", sans-serif;\n        color: var(--text-dark);\n        line-height: 1.6;\n    }\n\n    \/* 2. TOP SEKTION (Tabelle \u0026 Bild) *\/\n    .product-top-section {\n        display: flex;\n        flex-wrap: wrap;\n        gap: 30px;\n        align-items: flex-start; \n        justify-content: center;\n        margin-bottom: 30px;\n    }\n\n    .info-column {\n        flex: 1.5;\n        min-width: 280px;\n    }\n\n    .image-column {\n        flex: 1;\n        min-width: 250px;\n        display: flex;\n        justify-content: center;\n        align-items: flex-start;\n    }\n\n    .side-image {\n        max-width: 240px;\n        height: auto;\n        display: block;\n        margin-top: -25px; \/* Desktop-Lift *\/\n    }\n\n    \/* 3. 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AKKORDEON \u0026 INNEN-ÜBERSCHRIFTEN *\/\n    .accordion-container {\n        border-top: 1px solid var(--border-light);\n        margin: 20px 0;\n    }\n    .accordion-item {\n        border-bottom: 1px solid var(--border-light);\n    }\n    .accordion-header {\n        width: 100%;\n        padding: 20px 0;\n        background: none;\n        border: none;\n        display: flex;\n        justify-content: space-between;\n        align-items: center;\n        font-family: inherit;\n        font-size: 25px;\n        color: var(--primary-color) !important; \/* Türkis für Haupt-Header *\/\n        text-transform: uppercase;\n        cursor: pointer;\n        text-align: left;\n    }\n\n    \/* Styling für h4 Überschriften im Scientific Context *\/\n    .content-inner h4 {\n        color: var(--primary-color) !important;\n        text-transform: uppercase;\n        margin: 20px 0 10px 0;\n        font-size: 20px;\n    }\n\n    .icon { position: relative; width: 20px; height: 20px; flex-shrink: 0; margin-left: 10px; }\n    .icon::before, .icon::after {\n        content: '';\n        position: absolute;\n        background-color: currentColor;\n        transition: transform 0.3s ease;\n    }\n    .icon::before { width: 100%; height: 2px; top: 50%; left: 0; transform: translateY(-50%); }\n    .icon::after { width: 2px; height: 100%; left: 50%; top: 0; transform: translateX(-50%); }\n    .accordion-item.active .icon::after { transform: translateX(-50%) rotate(90deg); opacity: 0; }\n\n    .accordion-content {\n        max-height: 0;\n        overflow: hidden;\n        transition: max-height 0.5s cubic-bezier(0, 1, 0, 1);\n    }\n    .accordion-item.active .accordion-content {\n        max-height: 4000px;\n        transition: max-height 1s ease-in-out;\n    }\n    .content-inner { padding: 15px 0 35px 0; font-size: 19px; }\n\n    .sequence-box {\n        word-break: break-all;\n        font-family: monospace;\n        background: #f4f4f4;\n        padding: 15px;\n        font-size: 15px;\n        border-left: 4px solid var(--primary-color);\n        margin-top: 10px;\n    }\n\n    \/* 5. MOBILE ANPASSUNGEN *\/\n    @media (max-width: 600px) {\n        .product-top-section {\n            flex-direction: column;\n            align-items: center;\n        }\n        .image-column {\n            order: -1;\n            margin-bottom: 20px;\n            width: 100%;\n        }\n        .side-image {\n            margin-top: 0;\n            margin-left: auto;\n            margin-right: auto;\n        }\n        .custom-list { font-size: 17px; }\n        .accordion-header { font-size: 20px; }\n    }\n\/* 6. INTRO TEXT SEKTION *\/\n    .intro-section {\n        margin: 20px 0;\n        font-size: 19px; \/* Desktop Größe *\/\n        line-height: 1.8;\n    }\n\n    \/* Mobile Anpassung für den Intro-Text *\/\n    @media (max-width: 600px) {\n        .intro-section {\n            font-size: 17px; \n            text-align: left;\n            padding: 0 5px;\n        }\n    }\n  \n\u003c\/style\u003e\n\u003cdiv class=\"container\"\u003e\n\u003cdiv class=\"product-top-section\"\u003e\n\u003cdiv class=\"info-column\"\u003e\n\u003cul class=\"custom-list\"\u003e\n\u003cli\u003eCrystallizable\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/www.crystalsfirst.com\/smartsoak\/\" target=\"_blank\"\u003eSmartSoak\u003csup\u003e®\u003c\/sup\u003e\u003c\/a\u003e established\u003c\/li\u003e \n\u003cli\u003ePurity \u0026gt; 95%\u003c\/li\u003e \n\u003cli\u003eMelting temperature of 40°C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"image-column\"\u003e\u003cimg class=\"side-image\" alt=\"Quality Plus Siegel\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/Stempel-QUALITY-PLUS-new.png?v=1771953936\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003chr\u003e\n\u003cdiv class=\"intro-section\"\u003e\n\u003cp\u003eThe E6-associated protein (E6AP), encoded by the UBE3A gene, is a HECT-type E3 ligase that plays a critical role in the ubiquitin-proteasome pathway by recognizing specific target proteins and facilitating their ubiquitination. E6AP is an important therapeutic target, as its dysregulation is associated with several diseases, including cervical cancer and neurodevelopmental disorders such as Angelman syndrome.\u003cbr\u003e Our recombinant E6AP(HECT) protein is of high crystallography-grade quality and is well suited for use in biochemical and biophysical assays, structural analysis, protein crystallography and SmartSoak-enabled co-structure determination.\n\n\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003econstruct design\u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003eThe construct GGS_E6AP(Q495-L852) includes the catalytic HECT-domain spanning residues Q495-L852 comprising the C-lobe harboring the catalytic cysteine and the E2-binding site within the N-terminal lobe (N-lobe). The N-terminal GGS sequence remains from the TEV cleavage site.\n\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eProtein Properties \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003e\u003cb\u003eProtein Construct:\u003c\/b\u003e GGS_E6AP(Q495-L852) \u003cbr\u003e\u003cb\u003eSource:\u003c\/b\u003e Human\u003cbr\u003e\u003cb\u003eExpression Host:\u003c\/b\u003e \u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cbr\u003e\u003cb\u003eMolecular weight [kDa]:\u003c\/b\u003e 41.7\u003cbr\u003e\u003cb\u003epI:\u003c\/b\u003e 5.1\u003cbr\u003e\u003cb\u003eExtinction Coefficient [M\u003csup\u003e-1\u003c\/sup\u003ecm\u003csup\u003e-1\u003c\/sup\u003e]:\u003c\/b\u003e 33350\u003cbr\u003e\u003cb\u003eTag:\u003c\/b\u003e none\u003c\/p\u003e\n\u003cp style=\"margin-top: 15px;\"\u003e\u003cb\u003eAmino acid sequence:\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"sequence-box\"\u003eGGSQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRDSVLIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eQuality Plus \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003e\u003cb\u003eQC Data:\u003c\/b\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/E6AP_datasheet_NewVersion_2.pdf?v=1786535205\" target=\"_blank\"\u003eDatasheet\u003c\/a\u003e\u003cbr\u003e\u003cb\u003ePurity SDS:\u003c\/b\u003e \u0026gt;95 % \u003cbr\u003e\u003cb\u003eHydrodynamic Radius (nm):\u003c\/b\u003e 4.2\u003cbr\u003e\u003cb\u003ePolydispersity Index (PDI): \u003c\/b\u003e 0.67\u003cbr\u003e\u003cb\u003eT\u003csub\u003eon\u003c\/sub\u003e (°C):\u003c\/b\u003e 37.6\u003cbr\u003e\u003cb\u003eT\u003csub\u003em\u003c\/sub\u003e (°C):\u003c\/b\u003e 40.1\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eScientific context \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003ch4\u003eStructure and function\u003c\/h4\u003e\n\u003cp\u003eThe E3 ligase E6AP belongs to the HECT (homologous to the E6AP C-terminus) family of E3 ligases, one of the three major classes of E3 ligases, alongside RING (really interesting new gene) and RBR (RING-between-RING) E3 ligases. The N-terminal region of full-length E6AP provides a platform for the binding of substrates targeted for ubiquitination, as well as regulatory proteins that modulate E6AP activity, such as the human papillomavirus (HPV) E6 protein and HERC2. In addition, many protein-protein interactions involving E6AP are mediated by an LxxLL motif (where x is any amino acid). \u003cbr\u003e\nThe C-terminal HECT domain adopts a characteristic L-shaped architecture comprising two distinct subdomains. It consists of a large N-lobe, which recruits E2-ubiquitin complexes, and a smaller C-lobe, which contains the catalytic cysteine responsible for the transfer of ubiquitin to lysine residues on the substrate.\n\u003c\/p\u003e\n\u003cbr\u003e\n\u003ch4\u003eE6AP as an important drug target\u003c\/h4\u003e\n\u003cp\u003eSeveral human diseases are associated with dysregulation of E6AP activity. The E3 ligase was first linked to cervical cancer when it was found to be hijacked by the HPV oncoprotein E6. Formation of the E6AP\/E6 complex leads to the ubiquitination and subsequent degradation of the tumor suppressor p53, an important step in the development of cervical cancer (Scheffner et al., 1993). \u003cbr\u003e\nAdditionally, E6AP is associated with a variety of neurological disorders, including Angelman syndrome (AS), which results from loss or reduced expression of the maternally inherited UBE3A allele. In contrast, increased UBE3A expression has been associated with autism spectrum disorder (ASD). \n\u003c\/p\u003e\n\u003cbr\u003e\n\u003cdiv style=\"font-size: 15px; margin-top: 25px; border-top: 1px dotted #ccc; padding-top: 15px;\"\u003e\n\u003cb\u003eReferences:\u003c\/b\u003e\u003cbr\u003eWang, Z., Fan, F., Li, Z. et al. \u003cb\u003e(2024)\u003c\/b\u003e. Structural insights into the functional mechanism of the ubiquitin ligase E6AP.\u003cb\u003eNature communications.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/www.nature.com\/articles\/s41467-024-47586-w\" target=\"_blank\"\u003e10.1038\/s41467-024-47586-w\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003eScheffner, M.; Huibregtse, J. M.; Vierstra, R. D.; Howley, P. M. \u003cb\u003e(1993)\u003c\/b\u003e. The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53. \u003cb\u003eCell.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/www.cell.com\/cell\/abstract\/0092-8674(93)90384-3\" target=\"_blank\"\u003e10.1016\/0092-8674(93)90384-3\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003eOwais, A., Mishra, R. K., Kiyokawa, H. \u003cb\u003e(2020)\u003c\/b\u003e.  The HECT E3 Ligase E6AP\/UBE3A as a Therapeutic Target in Cancer and Neurological Disorders.\u003cb\u003eCancers.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/www.mdpi.com\/2072-6694\/12\/8\/2108\" target=\"_blank\"\u003e10.3390\/cancers12082108\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n","brand":"CrystalsFirst Shop","offers":[{"title":"1 mg","offer_id":57620526727551,"sku":null,"price":3260.0,"currency_code":"EUR","in_stock":true},{"title":"5 mg","offer_id":57620526760319,"sku":null,"price":15000.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/E6APTrimer_BU_1b8afed0-736a-4ffb-8016-fe157908bb52.png?v=1786525241","url":"https:\/\/shop.crystalsfirst.com\/products\/soon-available-br-recombinant-b-e6ap-b-konstukt-x-domain-voller-name","provider":"CrystalsFirst GmbH","version":"1.0","type":"link"}