{"product_id":"recombinant-trim21-e3-ligase","title":"SOON AVAILABLE: \u003cbr\u003e Recombinant \u003cb\u003eTRIM21\u003c\/b\u003e(V287-L465), PRYSPRY domain, Tripartite motif-containing 21 protein, E3 Ligase","description":"\u003cp\u003e\u003cmeta charset=\"UTF-8\"\u003e \u003cmeta name=\"viewport\" content=\"width=device-width, initial-scale=1.0\"\u003e\u003c\/p\u003e\n\u003cstyle\u003e\n    \/* 1. VARIABLEN \u0026 RESET *\/\n    :root {\n        --primary-color: #0dbaad;\n        --text-dark: #373636;\n        --border-light: #e0e0e0;\n    }\n\n    * { margin: 0; padding: 0; box-sizing: border-box; }\n\n    .container {\n        max-width: 1200px;\n        margin: 0 auto;\n        background-color: white;\n        padding: 20px;\n        font-family: \"Source Sans Pro\", sans-serif;\n        color: var(--text-dark);\n        line-height: 1.6;\n    }\n\n    \/* 2. TOP SEKTION (Tabelle \u0026 Bild) *\/\n    .product-top-section {\n        display: flex;\n        flex-wrap: wrap;\n        gap: 30px;\n        align-items: flex-start; \n        justify-content: center;\n        margin-bottom: 30px;\n    }\n\n    .info-column {\n        flex: 1.5;\n        min-width: 280px;\n    }\n\n    .image-column {\n        flex: 1;\n        min-width: 250px;\n        display: flex;\n        justify-content: center;\n        align-items: flex-start;\n    }\n\n    .side-image {\n        max-width: 240px;\n        height: auto;\n        display: block;\n        margin-top: -25px; \/* Desktop-Lift *\/\n    }\n\n    \/* 3. 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AKKORDEON \u0026 INNEN-ÜBERSCHRIFTEN *\/\n    .accordion-container {\n        border-top: 1px solid var(--border-light);\n        margin: 20px 0;\n    }\n    .accordion-item {\n        border-bottom: 1px solid var(--border-light);\n    }\n    .accordion-header {\n        width: 100%;\n        padding: 20px 0;\n        background: none;\n        border: none;\n        display: flex;\n        justify-content: space-between;\n        align-items: center;\n        font-family: inherit;\n        font-size: 25px;\n        color: var(--primary-color) !important; \/* Türkis für Haupt-Header *\/\n        text-transform: uppercase;\n        cursor: pointer;\n        text-align: left;\n    }\n\n    \/* Styling für h4 Überschriften im Scientific Context *\/\n    .content-inner h4 {\n        color: var(--primary-color) !important;\n        text-transform: uppercase;\n        margin: 20px 0 10px 0;\n        font-size: 20px;\n    }\n\n    .icon { position: relative; width: 20px; height: 20px; flex-shrink: 0; margin-left: 10px; }\n    .icon::before, .icon::after {\n        content: '';\n        position: absolute;\n        background-color: currentColor;\n        transition: transform 0.3s ease;\n    }\n    .icon::before { width: 100%; height: 2px; top: 50%; left: 0; transform: translateY(-50%); }\n    .icon::after { width: 2px; height: 100%; left: 50%; top: 0; transform: translateX(-50%); }\n    .accordion-item.active .icon::after { transform: translateX(-50%) rotate(90deg); opacity: 0; }\n\n    .accordion-content {\n        max-height: 0;\n        overflow: hidden;\n        transition: max-height 0.5s cubic-bezier(0, 1, 0, 1);\n    }\n    .accordion-item.active .accordion-content {\n        max-height: 4000px;\n        transition: max-height 1s ease-in-out;\n    }\n    .content-inner { padding: 15px 0 35px 0; font-size: 19px; }\n\n    .sequence-box {\n        word-break: break-all;\n        font-family: monospace;\n        background: #f4f4f4;\n        padding: 15px;\n        font-size: 15px;\n        border-left: 4px solid var(--primary-color);\n        margin-top: 10px;\n    }\n\n    \/* 5. MOBILE ANPASSUNGEN *\/\n    @media (max-width: 600px) {\n        .product-top-section {\n            flex-direction: column;\n            align-items: center;\n        }\n        .image-column {\n            order: -1;\n            margin-bottom: 20px;\n            width: 100%;\n        }\n        .side-image {\n            margin-top: 0;\n            margin-left: auto;\n            margin-right: auto;\n        }\n        .custom-list { font-size: 17px; }\n        .accordion-header { font-size: 20px; }\n    }\n\/* 6. INTRO TEXT SEKTION *\/\n    .intro-section {\n        margin: 20px 0;\n        font-size: 19px; \/* Desktop Größe *\/\n        line-height: 1.8;\n    }\n\n    \/* Mobile Anpassung für den Intro-Text *\/\n    @media (max-width: 600px) {\n        .intro-section {\n            font-size: 17px; \n            text-align: left;\n            padding: 0 5px;\n        }\n    }\n  \n\u003c\/style\u003e\n\u003cdiv class=\"container\"\u003e\n\u003cdiv class=\"product-top-section\"\u003e\n\u003cdiv class=\"info-column\"\u003e\n\u003cul class=\"custom-list\"\u003e\n\u003cli\u003ePurity \u0026gt; 95%\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"image-column\"\u003e\u003cimg class=\"side-image\" alt=\"Quality Plus Siegel\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/Stempel-QUALITY-PLUS-new.png?v=1771953936\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003chr\u003e\n\u003cdiv class=\"intro-section\"\u003e\n\u003cp\u003eThe human tripartite motif-containing 21 (TRIM21) protein is a ubiquitin E3 ligase that plays a critical role in the ubiquitin-proteasome pathway, specifically in the recognition of target proteins and their recruitment to the proteasome. The PRYSPRY domain of TRIM21 plays an important role in the recognition of antibody-coated pathogens and subsequent degradation by the proteasome, making it an important target in the development of bivalent molecules such as Proteolysis Targeting Chimeras (PROTACs). Our recombinant TRIM21(PRYSPRY) protein is of high quality and is well suited for researchers pursuing the development of novel PROTAC strategies.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003econstruct design\u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003eOur recombinant GSG_TRIM21(V287-L465) protein contains the C-terminal PRY-SPRY domain of TRIM21, which spans residues V287 - L465. The construct also contains an N-terminal GSG linker, which serves to optimize tag cleavage by a TEV Protease.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eProtein Properties \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003e\u003cb\u003eProtein Construct:\u003c\/b\u003e GSG_TRIM21(V287-L465) \u003cbr\u003e\u003cb\u003eSource:\u003c\/b\u003e Human\u003cbr\u003e\u003cb\u003eExpression Host:\u003c\/b\u003e \u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cbr\u003e\u003cb\u003eMolecular weight [kDa]:\u003c\/b\u003e 20.5\u003cbr\u003e\u003cb\u003epI:\u003c\/b\u003e 5.9\u003cbr\u003e\u003cb\u003eExtinction Coefficient [M\u003csup\u003e-1\u003c\/sup\u003ecm\u003csup\u003e-1\u003c\/sup\u003e]:\u003c\/b\u003e 43430\u003cbr\u003e\u003cb\u003eTag:\u003c\/b\u003e none\u003c\/p\u003e\n\u003cp style=\"margin-top: 15px;\"\u003e\u003cb\u003eAmino acid sequence:\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"sequence-box\"\u003eGSGVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPL\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eScientific context \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003ch4\u003eStructure and function\u003c\/h4\u003e\n\u003cp\u003eThe tripartite motif (TRIM) protein family includes around 70 proteins involved in a wide range of cellular functions such as cell growth, differentiation, and programmed cell death. The multidomain TRIM21 protein consists of an N-terminal RING finger domain with E3 ligase activity, a coiled-coil domain mediating oligomerization after ubiquitination of substrates targeted for degradation as well as a C-terminal PRYSPRY domain. The latter adopts a distorted beta-sandwich fold and features a canonical binding interface for the Fc region of immunoglobulins which coat pathogens that can be marked for proteasomal degradation.\u003c\/p\u003e\n\u003cbr\u003e\n\u003ch4\u003eTRIM21 as an important drug target\u003c\/h4\u003e\n\u003cp\u003eA key element of TRIM21’s activity is the PRYSPRY domain, as it enables binding to the Fc regions of immunoglobulins and subsequent ubiquitination and degradation of antibody-bound pathogens. The PRYSPRY domain remains a central target for therapeutic intervention, as it contains, in addition to two antibody-binding pockets, other smaller ligand binding pockets (Kim et al., 2025). The capacity of the TRIM21-PRYSPRY domain to break down pathogenic cargo bound to endogenous antibodies has been utilized in the so-called TRIM-AWAY technique. In this approach, introducing an antibody that targets a specific protein into cells triggers its degradation through TRIM21.\u003cbr\u003eThe development of bivalent molecules has also been successful in the field of anticancer therapeutics, where so-called “TRIMTACs”, direct TRIM21 to nuclear pore proteins, disrupting nuclear transport and leading to rapid cancer cell death (Scemama de Gialluly et al., 2025). It has also been discovered that TRIM21 preferentially ubiquitinates large, multimeric complexes in vivo, which makes it a potentially interesting E3 ligase for the development of PROTACs against protein aggregates.\u003cbr\u003eAdditionally, mutations in the PRYSPRY domain have been linked to autoimmune diseases like rheumatoid arthritis or systemic lupus erythematosus, making it an important target for autoimmune therapeutics.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cdiv style=\"font-size: 15px; margin-top: 25px; border-top: 1px dotted #ccc; padding-top: 15px;\"\u003e\n\u003cb\u003eReferences:\u003c\/b\u003e\u003cbr\u003e\n\nKim, Da Som, Park, Youngjae, Tsokos, George C., CHo, Mi-La, Kwok, Seung-Ki. \u003cb\u003e(2025)\u003c\/b\u003e. The Ubiquitin E3 ligase TRIM21 suppresses type I interferon signaling via STING degradation and ameliorates systemic autoimmunity.\u003cb\u003eNature.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/www.nature.com\/articles\/s12276-025-01490-5\" target=\"_blank\"\u003e10.1038\/s12276-025-01490-5\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\n  \nScemama de Gialluly, M.A., Allen, A.R., Hayes, E.H. et al. \u003cb\u003e(2025)\u003c\/b\u003e. Elaboration of molecular glues that target TRIM21 into TRIMTACs that degrade protein aggregates.\u003cb\u003eNature Communications.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/www.nature.com\/articles\/s41467-025-61818-7\" target=\"_blank\"\u003e10.1038\/s41467-025-61818-7\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\n  \nKim, Y., Lučić, A., Lenz, C. et al. \u003cb\u003e(2025)\u003c\/b\u003e. Crystallographic fragment screening reveals ligand hotspots in TRIM21 PRY-SPRY domain.\u003cb\u003eCommunications Chemistry.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/www.nature.com\/articles\/s42004-025-01574-3\" target=\"_blank\"\u003e10.1038\/s42004-025-01574-3\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\n  \n  \nJames, Leo C., Keeble, Anthony H., Khan, Zahra, Trowsdale, John. \u003cb\u003e(2007)\u003c\/b\u003e. Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function. \u003cb\u003eMol Neurodegeneration.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/www.pnas.org\/doi\/10.1073\/pnas.0609174104\" target=\"_blank\"\u003e 10.1073\/pnas.0609174104\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"CrystalsFirst Shop","offers":[{"title":"1 mg","offer_id":57512189231487,"sku":null,"price":1500.0,"currency_code":"EUR","in_stock":true},{"title":"5 mg","offer_id":57512189264255,"sku":null,"price":6750.0,"currency_code":"EUR","in_stock":true},{"title":"10 mg","offer_id":57512189297023,"sku":null,"price":12000.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/TRIM_21.jpg?v=1779093114","url":"https:\/\/shop.crystalsfirst.com\/products\/recombinant-trim21-e3-ligase","provider":"CrystalsFirst GmbH","version":"1.0","type":"link"}