{"product_id":"recombinant-tead3r218-d435","title":"Recombinant \u003cb\u003eTEAD3\u003c\/b\u003e (R218-D435) YAP-binding domain, Transcriptional Enhanced Associate Domain 3, Transcriptional enhancer factor TEF-5","description":"\u003cp\u003e\u003cmeta charset=\"UTF-8\"\u003e \u003cmeta content=\"width=device-width, initial-scale=1.0\" name=\"viewport\"\u003e\u003c\/p\u003e\n\u003cstyle\u003e\n    \/* 1. VARIABLEN \u0026 RESET *\/\n    :root {\n        --primary-color: #0dbaad;\n        --text-dark: #373636;\n        --border-light: #e0e0e0;\n    }\n\n    * { margin: 0; padding: 0; box-sizing: border-box; }\n\n    .container {\n        max-width: 1200px;\n        margin: 0 auto;\n        background-color: white;\n        padding: 20px;\n        font-family: \"Source Sans Pro\", sans-serif;\n        color: var(--text-dark);\n        line-height: 1.6;\n    }\n\n    \/* 2. 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AKKORDEON \u0026 INNEN-ÜBERSCHRIFTEN *\/\n    .accordion-container {\n        border-top: 1px solid var(--border-light);\n        margin: 20px 0;\n    }\n    .accordion-item {\n        border-bottom: 1px solid var(--border-light);\n    }\n    .accordion-header {\n        width: 100%;\n        padding: 20px 0;\n        background: none;\n        border: none;\n        display: flex;\n        justify-content: space-between;\n        align-items: center;\n        font-family: inherit;\n        font-size: 25px;\n        color: var(--primary-color) !important; \/* Türkis für Haupt-Header *\/\n        text-transform: uppercase;\n        cursor: pointer;\n        text-align: left;\n    }\n\n    \/* Styling für h4 Überschriften im Scientific Context *\/\n    .content-inner h4 {\n        color: var(--primary-color) !important;\n        text-transform: uppercase;\n        margin: 20px 0 10px 0;\n        font-size: 20px;\n    }\n\n    .icon { position: relative; width: 20px; height: 20px; flex-shrink: 0; margin-left: 10px; }\n    .icon::before, .icon::after {\n        content: '';\n        position: absolute;\n        background-color: currentColor;\n        transition: transform 0.3s ease;\n    }\n    .icon::before { width: 100%; height: 2px; top: 50%; left: 0; transform: translateY(-50%); }\n    .icon::after { width: 2px; height: 100%; left: 50%; top: 0; transform: translateX(-50%); }\n    .accordion-item.active .icon::after { transform: translateX(-50%) rotate(90deg); opacity: 0; }\n\n    .accordion-content {\n        max-height: 0;\n        overflow: hidden;\n        transition: max-height 0.5s cubic-bezier(0, 1, 0, 1);\n    }\n    .accordion-item.active .accordion-content {\n        max-height: 4000px;\n        transition: max-height 1s ease-in-out;\n    }\n    .content-inner { padding: 15px 0 35px 0; font-size: 19px; }\n\n    .sequence-box {\n        word-break: break-all;\n        font-family: monospace;\n        background: #f4f4f4;\n        padding: 15px;\n        font-size: 15px;\n        border-left: 4px solid var(--primary-color);\n        margin-top: 10px;\n    }\n\n    \/* 5. MOBILE ANPASSUNGEN *\/\n    @media (max-width: 600px) {\n        .product-top-section {\n            flex-direction: column;\n            align-items: center;\n        }\n        .image-column {\n            order: -1;\n            margin-bottom: 20px;\n            width: 100%;\n        }\n        .side-image {\n            margin-top: 0;\n            margin-left: auto;\n            margin-right: auto;\n        }\n        .custom-list { font-size: 17px; }\n        .accordion-header { font-size: 20px; }\n    }\n\/* 6. INTRO TEXT SEKTION *\/\n    .intro-section {\n        margin: 20px 0;\n        font-size: 19px; \/* Desktop Größe *\/\n        line-height: 1.8;\n    }\n\n    \/* Mobile Anpassung für den Intro-Text *\/\n    @media (max-width: 600px) {\n        .intro-section {\n            font-size: 17px; \n            text-align: left;\n            padding: 0 5px;\n        }\n    }\n  \n\u003c\/style\u003e\n\u003cdiv class=\"container\"\u003e\n\u003cdiv class=\"product-top-section\"\u003e\n\u003cdiv class=\"info-column\"\u003e\n\u003cul class=\"custom-list\"\u003e\n\u003cli\u003ePurity \u0026gt; 95%\u003c\/li\u003e\n\u003cli\u003eMonodisperse\u003c\/li\u003e\n\u003cli\u003eMelting temperature of 63°C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"image-column\"\u003e\u003cimg class=\"side-image\" alt=\"Quality Plus Siegel\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/Stempel-QUALITY-PLUS-new.png?v=1771953936\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003chr\u003e\n\u003cdiv class=\"intro-section\"\u003e\n\u003cp\u003eThe Transcriptional Enhanced Associate Domain 3 (TEAD3) protein is a transcription factor that interacts with the downstream effectors YAP and TAZ of the Hippo signaling pathway. Given the central role of Hippo signaling in regulating essential cellular processes, TEAD3 is also associated with a wide range of diseases, making its YAP-binding domain a particularly attractive target for therapeutic drug discovery. Our recombinant TEAD3(R218-D435) is of high quality and is well suited for researchers pursuing the development of therapeutics for targeted protein degradation and anti-cancer treatments targeting the YAP-binding domain.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003econstruct design\u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003eOur recombinant S_TEAD3(R218-D435) protein contains the C-terminal YAP-binding domain of the human transcription factor TEAD3. It has an additional N-terminal Serine, which remains from the TEV cleavage site.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eProtein Properties \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003e\u003cb\u003eProtein Construct:\u003c\/b\u003e TEAD3(R218-D435) \u003cbr\u003e\u003cb\u003eSource:\u003c\/b\u003e Human\u003cbr\u003e\u003cb\u003eExpression Host:\u003c\/b\u003e \u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cbr\u003e\u003cb\u003eMolecular weight [kDa]:\u003c\/b\u003e 25.1\u003cbr\u003e\u003cb\u003epI:\u003c\/b\u003e 6.7\u003cbr\u003e\u003cb\u003eExtinction Coefficient [M\u003csup\u003e-1\u003c\/sup\u003ecm\u003csup\u003e-1\u003c\/sup\u003e]:\u003c\/b\u003e 21890\u003cbr\u003e\u003cb\u003eConcentration:\u003c\/b\u003e 10 mg\/mL\u003cbr\u003e\u003cb\u003eStorage Buffer:\u003c\/b\u003e 20 mM Tris pH 7.5, 150 mM NaCl, 5% Glycerol, 2 mM DTT \u003cbr\u003e\u003cb\u003eTag:\u003c\/b\u003e none\u003c\/p\u003e\n\u003cp style=\"margin-top: 15px;\"\u003e\u003cb\u003eAmino acid sequence:\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"sequence-box\"\u003eSRTIASSRLRLLEYSAFMEVQRDPDTYSKHLFVHIGQTNPAFSDPPLEAVDVRQIYDKFPEKKGGLKELYEKGPPNAFFLVKFWADLNSTIQEGPGAFYGVSSQYSSADSMTISVSTKVCSFGKQVVEKVETEYARLENGRFVYRIHRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTSRDSQETLLVIAFVFEVSTSEHGAQHHVYKLVKD\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eQuality Plus\u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003e\u003cb\u003eQC Data:\u003c\/b\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/TEAD3_datasheet_NewVersion.pdf?v=1778769607\" target=\"_blank\"\u003eDatasheet\u003c\/a\u003e\u003cbr\u003e\u003cb\u003ePurity SDS:\u003c\/b\u003e \u0026gt;95 % \u003cbr\u003e\u003cb\u003eHydrodynamic Radius (nm):\u003c\/b\u003e 2.78\u003cbr\u003e\u003cb\u003ePolydispersity Index (PDI): \u003c\/b\u003e 0.13\u003cbr\u003e\u003cb\u003eT\u003csub\u003em\u003c\/sub\u003e (°C):\u003c\/b\u003e 63.3\u003cbr\u003e\u003cb\u003eT\u003csub\u003eon\u003c\/sub\u003e (°C):\u003c\/b\u003e 56.9\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eScientific context \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003ch4\u003eStructure and function\u003c\/h4\u003e\n\u003cp\u003eTEAD3 is part of the TEAD transcription factor family that regulates important cellular functions like tissue homeostasis, cell growth and proliferation. TEAD3 drives gene expression via its highly conserved TEA DNA-binding domain, which folds into a three-helix bundle. The H3 helix incorporates the DNA recognition surface which features 3 serines essential for DNA binding activities. In addition TEAD3 has a C-terminal interaction domain, which enables binding to transcriptional cofactor Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ), which is obligatory for TEAD3 to regulate transcription. The C-terminal domain adopts an immunoglobulin-like ß-sandwich fold while the YAP itself is mostly unstructured and only forms secondary structure elements upon binding to TEAD3. Both TEA domain and C-terminal domain are connected via a proline-rich region, which is necessary for interaction of the C-terminal domain with YAP\/TAZ.\u003c\/p\u003e\n\u003cbr\u003e\n\u003ch4\u003eTEAD3 as an important drug target\u003c\/h4\u003e\n\u003cp\u003eTEAD3 is a transcription factor that functions downstream of the oncogenic co-activators YAP and TAZ, key effectors of the Hippo signaling pathway. The interaction with YAP\/TAZ, enables TEAD3 to bind DNA and regulates the expression of genes involved in proliferation, differentiation, and apoptosis. Hippo signaling controls gene expression largely through suppressing YAP and TAZ. In several types of cancer, however, dysregulation of Hippo signaling causes YAP\/TAZ accumulation and tumor progression, making inhibition of the interaction of TEAD3 with YAP a promising therapeutic strategy.\u003cbr\u003eOne interaction inhibition strategy involves a hydrophobic pocket in the C-terminal region which is under physiological conditions occupied by a palmitate molecule binding to a conserved cysteine. This pocket has been shown to be important for TEAD3 stability as well as the TEAD3-YAP interaction, leading to the development of several small molecule inhibitors targeting this site. Another TEAD3 inhibition strategy is targeting the transcription factor with Proteolysis-targeting chimeras (PROTACs), which recruit the protein to an E3 ligase, thereby inducing its ubiquitination and degradation by the proteasome.\u003cbr\u003eTEAD3 is not only an important target in cancer research but has also become the focus of reproductive biology due to its association with maternal-fetal immune tolerance. In addition, studies in neuroscience have provided initial evidence for the involvement of TEAD3 in neural development and have linked it to neurodegenerative diseases such as Alzheimer's disease.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cdiv style=\"font-size: 15px; margin-top: 25px; border-top: 1px dotted #ccc; padding-top: 15px;\"\u003e\n\u003cb\u003eReferences:\u003c\/b\u003e \u003cbr\u003eHodáková Z, Landin-Malt, A., Benhaddou, A., Zider, A., Flagiello, D..\u003cb\u003e(2016)\u003c\/b\u003e. An evolutionary, structural and functional overview of the mammalian TEAD1 and TEAD2 transcription factors. \u003cb\u003eGene.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27421669\/\" target=\"_blank\"\u003e10.1016\/j.gene.2016.07.028\u003c\/a\u003e \u003cbr\u003e\u003cbr\u003eKang, C., Xu, W., Song, H., Hong, W..\u003cb\u003e(2026)\u003c\/b\u003e. Illuminating Clinical TEAD Inhibitors Through Structural Biology.\u003cb\u003eJournal of Medicinal Chemistry.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.jmedchem.6c01021\" target=\"_blank\"\u003e10.1021\/acs.jmedchem.6c01021\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003eGu, B., Ferreira, L.M.R., Herrera, S., Brown, L., Lieberman, J., Sherwood, R.I., Meissner, T.B., Strominger, J..L. \u003cb\u003e(2025)\u003c\/b\u003e. The TEA domain transcription factors TEAD1 and TEAD3 and WNT signaling determine HLA-G expression in human extravillous trophoblasts.\u003cb\u003ePNAS.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/www.pnas.org\/doi\/10.1073\/pnas.2425339122\" target=\"_blank\"\u003e10.1073\/pnas.2425339122\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003eChen, D., Wigglesworth-Littlewood, S. \u0026amp; Gunn-Moore, F.J. \u003cb\u003e(2025)\u003c\/b\u003e. The Hippo signaling pathway as a therapeutic target in Alzheimer’s disease.\u003cb\u003eMol Neurodegeneration.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/link.springer.com\/article\/10.1186\/s13024-025-00891-4\" target=\"_blank\"\u003e10.1186\/s13024-025-00891-4\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"CrystalsFirst Shop","offers":[{"title":"1 mg","offer_id":57495989191039,"sku":null,"price":1390.0,"currency_code":"EUR","in_stock":true},{"title":"5 mg","offer_id":57495989223807,"sku":null,"price":6600.0,"currency_code":"EUR","in_stock":true},{"title":"10 mg","offer_id":57495989256575,"sku":null,"price":11390.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/TEAD3_structure.jpg?v=1778837917","url":"https:\/\/shop.crystalsfirst.com\/products\/recombinant-tead3r218-d435","provider":"CrystalsFirst GmbH","version":"1.0","type":"link"}