{"product_id":"recombinant-b-cmet-b-r218-d435-yap-binding-domain-transcriptional-enhanced-associate-domain-3-transcriptional-enhancer-factor-tef-5-kopie","title":"Recombinant \u003cb\u003ec-MET\u003c\/b\u003e (N1049-G1346), receptor tyrosine kinase domain, cellular-mesenchymal epithelial transition factor","description":"\u003cp\u003e\u003cmeta charset=\"UTF-8\"\u003e \u003cmeta content=\"width=device-width, initial-scale=1.0\" name=\"viewport\"\u003e\u003c\/p\u003e\n\u003cstyle\u003e\n    \/* 1. VARIABLEN \u0026 RESET *\/\n    :root {\n        --primary-color: #0dbaad;\n        --text-dark: #373636;\n        --border-light: #e0e0e0;\n    }\n\n    * { margin: 0; padding: 0; box-sizing: border-box; }\n\n    .container {\n        max-width: 1200px;\n        margin: 0 auto;\n        background-color: white;\n        padding: 20px;\n        font-family: \"Source Sans Pro\", sans-serif;\n        color: var(--text-dark);\n        line-height: 1.6;\n    }\n\n    \/* 2. 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MOBILE ANPASSUNGEN *\/\n    @media (max-width: 600px) {\n        .product-top-section {\n            flex-direction: column;\n            align-items: center;\n        }\n        .image-column {\n            order: -1;\n            margin-bottom: 20px;\n            width: 100%;\n        }\n        .side-image {\n            margin-top: 0;\n            margin-left: auto;\n            margin-right: auto;\n        }\n        .custom-list { font-size: 17px; }\n        .accordion-header { font-size: 20px; }\n    }\n\/* 6. INTRO TEXT SEKTION *\/\n    .intro-section {\n        margin: 20px 0;\n        font-size: 19px; \/* Desktop Größe *\/\n        line-height: 1.8;\n    }\n\n    \/* Mobile Anpassung für den Intro-Text *\/\n    @media (max-width: 600px) {\n        .intro-section {\n            font-size: 17px; \n            text-align: left;\n            padding: 0 5px;\n        }\n    }\n  \n\u003c\/style\u003e\n\u003cdiv class=\"container\"\u003e\n\u003cdiv class=\"product-top-section\"\u003e\n\u003cdiv class=\"info-column\"\u003e\n\u003cul class=\"custom-list\"\u003e\n\u003cli\u003eCrystallizable\u003c\/li\u003e\n\u003cli\u003ePurity \u0026gt; 95%\u003c\/li\u003e\n\u003cli\u003eMonodisperse\u003c\/li\u003e\n\u003cli\u003eMelting temperature of 49°C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"image-column\"\u003e\u003cimg class=\"side-image\" alt=\"Quality Plus Siegel\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/Stempel-QUALITY-PLUS-new.png?v=1771953936\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003chr\u003e\n\u003cdiv class=\"intro-section\"\u003e\n\u003cp\u003eThe receptor tyrosine kinase c-MET is primarily expressed on epithelial cells and plays an important role in embryogenesis, wound healing, and tissue regeneration. Binding of its endogenous ligand, hepatocyte growth factor (HGF), activates c-MET by promoting receptor dimerization and autophosphorylation, generating docking sites for downstream signalling molecules. This pathway is tightly regulated under physiological conditions; however, these regulatory mechanisms can become disrupted in malignant cells, resulting in persistent c-MET activation which can contribute to tumor growth and metastasis. \u003c\/p\u003e\n\u003cp\u003eOur recombinant c-MET tyrosine kinase domain is of high crystallography-grade quality and is well suited for structural and biochemical investigations as well as therapeutic drug discovery efforts targeting c-MET in cancer.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003econstruct design\u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003eOur c-MET construct spans residues N1049-G1346 and comprises the receptor tyrosine kinase domain, including the two activation-loop phosphorylation sites Tyr1234 and Tyr1235. In addition, the construct contains the ATP-binding pocket, the catalytic loop and the C-terminal regulatory tail.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eProtein Properties \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003e\u003cb\u003eProtein Construct:\u003c\/b\u003e c-MET(N1049-G1346) \u003cbr\u003e\u003cb\u003eSource:\u003c\/b\u003e Human\u003cbr\u003e\u003cb\u003eExpression Host:\u003c\/b\u003e \u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cbr\u003e\u003cb\u003eMolecular weight [kDa]:\u003c\/b\u003e 33.7\u003cbr\u003e\u003cb\u003epI:\u003c\/b\u003e 8.45\u003cbr\u003e\u003cb\u003eExtinction Coefficient [M\u003csup\u003e-1\u003c\/sup\u003ecm\u003csup\u003e-1\u003c\/sup\u003e]:\u003c\/b\u003e 36900\u003cbr\u003e\u003cb\u003eConcentration:\u003c\/b\u003e 10.1 mg\/mL\u003cbr\u003e\u003cb\u003eStorage Buffer:\u003c\/b\u003e 20 mM Tris-HCl pH 8.3, 100 mM NaCl, 10 % glycerol, 1 mM TCEP \u003cbr\u003e\u003cb\u003eTag:\u003c\/b\u003e none\u003c\/p\u003e\n\u003cp style=\"margin-top: 15px;\"\u003e\u003cb\u003eAmino acid sequence:\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"sequence-box\"\u003eNTVHIDLSALNPELVQAVQHVVIGPSSLIVHFNEVIGRGHFGCVYHGTLLDNDGKKIHCAVKSLNRITDIGEVSQFLTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMKYLASKKFVHRDLAARNCMLDEKFTVKVADFGLARDMYDKEYYSVHNKTGAKLPVKWMALESLQTQKFTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVMLKCWHPKAEMRPSFSELVSRISAIFSTFIG\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eQuality Plus\u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003cp\u003e\u003cb\u003eQC Data:\u003c\/b\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/cMET_datasheet_NewVersion.pdf?v=1787931982\" target=\"_blank\"\u003eDatasheet\u003c\/a\u003e\u003cbr\u003e\u003cb\u003ePurity SDS:\u003c\/b\u003e \u0026gt;95 % \u003cbr\u003e\u003cb\u003eHydrodynamic Radius (nm):\u003c\/b\u003e 3.46\u003cbr\u003e\u003cb\u003ePolydispersity Index (PDI): \u003c\/b\u003e 0.18\u003cbr\u003e\u003cb\u003eT\u003csub\u003em\u003c\/sub\u003e (°C):\u003c\/b\u003e 49.3\u003cbr\u003e\u003cb\u003eT\u003csub\u003eon\u003c\/sub\u003e (°C):\u003c\/b\u003e 42.2\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion-item\"\u003e\n\u003cbutton class=\"accordion-header\"\u003eScientific context \u003cspan class=\"icon\"\u003e\u003c\/span\u003e\u003c\/button\u003e\n\u003cdiv class=\"accordion-content\"\u003e\n\u003cdiv class=\"content-inner\"\u003e\n\u003ch4\u003eStructure and function\u003c\/h4\u003e\n\u003cp\u003eThe receptor tyrosine kinase c-MET is a key component of the HGF\/c-MET signaling pathway and plays important roles in embryogenesis, wound healing, tissue regeneration, and cell proliferation. c-MET is primarily expressed on epithelial cells and is activated by binding of its endogenous ligand, hepatocyte growth factor (HGF). HGF-induced receptor dimerization promotes c-MET autophosphorylation and activation of its intracellular tyrosine kinase domain, which catalyzes the transfer of phosphate groups from ATP to specific tyrosine residues. These phosphorylation events create docking sites for downstream signaling proteins and initiate signaling pathways that regulate cellular processes such as proliferation and survival.\u003c\/p\u003e\n\u003cbr\u003e\n\u003ch4\u003eThe tyrosine kinase domain of c-MET as an important drug target\u003c\/h4\u003e\n\u003cp\u003eUnder physiological conditions, c-MET activity is tightly controlled by negative feedback mechanisms that prevent excessive signaling. Following activation, c-MET can undergo ubiquitination and be internalized through endocytosis, followed by transport to lysosomes for degradation. In malignant tumor cells, these regulatory mechanisms can become disrupted, resulting in constitutive activation of c-MET that contributes to tumor growth and cancer progression.\n\u003c\/p\u003e\n\u003cp\u003e\nTherapeutic strategies targeting c-MET include small-molecule tyrosine kinase inhibitors, monoclonal antibodies, and proteolysis-targeting chimeras (PROTACs). Small-molecule c-MET inhibitors can be classified according to their binding mode within the tyrosine kinase domain. Type I inhibitors bind to the ATP-binding site in the active kinase conformation, whereas Type II inhibitors engage the ATP-binding site and an adjacent allosteric pocket, stabilizing an inactive kinase conformation.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cdiv style=\"font-size: 15px; margin-top: 25px; border-top: 1px dotted #ccc; padding-top: 15px;\"\u003e\n\u003cb\u003eReferences:\u003c\/b\u003e \u003cbr\u003eZhu, C., Li, Y., Xu, H. et al. \u003cb\u003e(2026)\u003c\/b\u003e. C-MET tyrosine kinase receptor: mechanisms, clinical applications and future perspectives in cancer therapy. \u003cb\u003eNature.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/www.nature.com\/articles\/s41392-026-02682-9\" target=\"_blank\"\u003e10.1038\/s41392-026-02682-9\u003c\/a\u003e \u003cbr\u003e\u003cbr\u003eUchikawa, E., Chen, Z., Xiao, G.Y. et al.\u003cb\u003e(2021)\u003c\/b\u003e. Structural basis of the activation of c-MET receptor. \u003cb\u003eNature communications.\u003c\/b\u003e doi: \u003ca href=\"https:\/\/www.nature.com\/articles\/s41467-021-24367-3\" target=\"_blank\"\u003e10.1038\/s41467-021-24367-3 \u003c\/a\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"CrystalsFirst Shop","offers":[{"title":"1 mg","offer_id":58181053776255,"sku":null,"price":1870.0,"currency_code":"EUR","in_stock":true},{"title":"5 mg","offer_id":58181053809023,"sku":null,"price":8800.0,"currency_code":"EUR","in_stock":true},{"title":"10 mg","offer_id":58181053841791,"sku":null,"price":16500.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0958\/0612\/8511\/files\/cMET_Kinasedomain_BU.png?v=1787323859","url":"https:\/\/shop.crystalsfirst.com\/products\/recombinant-b-cmet-b-r218-d435-yap-binding-domain-transcriptional-enhanced-associate-domain-3-transcriptional-enhancer-factor-tef-5-kopie","provider":"CrystalsFirst GmbH","version":"1.0","type":"link"}